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An improved biochemical method for the analysis of HLA-class I antigens. Definition of new HLA-class I subtypes

✍ Scribed by Neefjes, Jacques J.; Breur-Vriesendorp, Birgitta S.; van Seventer, Gijs A.; Iványi, Pavol; Ploegh, Hidde L.


Book ID
123299351
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
820 KB
Volume
16
Category
Article
ISSN
0198-8859

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A simple sandwich ELISA method has been developed for the quantification of soluble HLA class I antigens (s-HLA) in human serum. The assay utilizes the monoclonal antibody Q6164, directed to a monomorphic determinant of the HLA a-chain, to capture the antigen and the biotinylated NAMB1 monoclonal an