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An improved automated periodate-resorcinol method for the determination of sialic acid

โœ Scribed by Theodore R. Oegema Jr.; Katherine M. Cooper


Book ID
107711833
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
428 KB
Volume
133
Category
Article
ISSN
0003-2697

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An improved fluorometric HPLC method for sialic acid determination was developed by employing synthetic N-propionylneuraminic acid (NPNA) as an internal standard. A fixed amount of NPNA was added to a sialoglycoconjugate sample. After hydrolyzing sialioglycoconjugates with diluted sulfuric acid, the