✦ LIBER ✦

An immunological assessment of lysosomal enzymes and other macromolecules sulfated during vegetative growth of Dictyostelium discoideum

✍ Scribed by Simon J. Davis; John F. Wheldrake; Hudson H. Freeze

Book ID: 102303150
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 219 KB
Volume: 38
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240380205

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✦ Synopsis

Western blotting and immunoprecipitation data indicated that lysosomal enzymes represent a subset of the sulfated macromolecules present in vegetative Dictyosteliurn discoideum amoebae and account for less than 2.5% of the total sulfate incorporated during vegetative growth. These data suggest that the majority of the highly sulfated macromolecules of vegetative D . discoideum amoebae are not related to the lysosomal enzymes.

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Oligosaccharide heterogeneity of glycopr

Oligosaccharide heterogeneity of glycoproteins sulfated during the vegetative growth of Dictyostelium discoideum

✍ Simon J. Davis 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 572 KB

Macromolecules are sulfated during the vegetative growth of Dictyostelium discoideum. A characterisation of the structures of sulfated oligosaccharides associated with these macromolecules indicates that the oligosaccharides are heterogeneous. Endoglycosidase and pronase digestion were used with gel