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An Immunoenzyme Linked Assay (ELISA) for the Detection of Antibodies to Truncated Glycoprotein D (tgD) of Bovine Herpesvirus-1

โœ Scribed by S. Petrini; M. Ferrari; S. Vincenzetti; A. Vita; A. Amici; G. Ramadori


Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
42 KB
Volume
30
Category
Article
ISSN
0165-7380

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A 3-step enzyme-linked immunosorbent assay (ELISA) was developed for detecting IgA antibodies to purified Epstein-Barr virus (EBV) polypeptides. The 3-step procedure included the use of a mouse anti-human IgA monoclonal antibody (MAb) to amplify the IgA reaction. The 2 major EBV proteins used in thi