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An immunochemical method for the detection of the expression of human gene loci in human-rodent somatic cell hybrids with special reference to theGPIlocus

✍ Scribed by Helen R. Palser; Phyllis J. McAlpine

Publisher: Springer
Year: 1976
Tongue: English
Weight: 526 KB
Volume: 14
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00485844

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✦ Synopsis

Species-specific antibodies, prepared against unpurified human and Chinese hamster fibroblast extracts, were used to identify the parental origins of enzymes in human-hamster somatic cell hybrids. Results of the detection of the expression of the human glucosephosphate isomerase gene locus (GPI) by eleetrophoretic and immunochemical techniques were concordant in 17 instances. The human GPI synthesized by fibroblasts derived from skin explants and by somatic cell hybrids retaining the human GPI locus, regardless of whether the human parental cells were lymphoeytes or fibroblasts, appeared to be antigenically identical.

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