An immunoaffinity column with a monoclonal antibody as ligand for human follicle stimulating hormone

✍ Scribed by Marlene Zandian; Alois Jungbauer

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 642 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900103

✦ Synopsis

Abstract

A detailed characterization regarding the chromatographic and biochemical properties of an immunoaffinity column for sample pretreatment using a follicle stimulating hormone (FSH) β‐unit specific mAb has been performed. A maximum binding capacity of approximately 300 μg/mL was determined. A K~D~ value of 4.5×10^–8^ M could be obtained in batch isotherm measurements. The dynamic binding capacity at 10% did not change when residence time was at least 6 min. High selectivity could be demonstrated using crude culture supernatant from FSH producing Chinese hamster ovary (CHO) cells. A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity.