AN ICAM-1 LIKE CELL ADHESION MOLECULE IS RESPONSIBLE FOR CD34 POSITIVE HAEMOPOIETIC STEM CELLS ADHESION TO BONE-MARROW STROMA
✍ Scribed by S.G.A. RAO; V.S. CHITNIS; A. DEORA; V. TANAVDE; S.S. DESAI
- Book ID
- 102966181
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 585 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
The microenvironment in the haematopoietic organs plays an important role in regulating and sustaining differentiation and self-renewal of haematopoietic stem cells. Although crucial for stem cell maintenance and homing, the stromal cell-stem cell interactions are poorly understood. Here we show that an ICAM-like molecule is responsible for stem cell adhesion to stromal cells in vitro. The molecule was characterized by a monoclonal antibody 3E 10 . Immunoblotting results indicated that the molecule had an electrophoretic mobility equal to that of intercellular cell adhesion molecule-1 (ICAM-1). Binding inhibition assays, however, showed that inhibition of binding of enriched CD34 cells by 3E 10 was more prominent in comparison with that of ICAM-1.