An HPLC assay for the norditerpenoid alkaloid methyllycaconitine, a potent nicotinic acetylcholine receptor antagonist

✍ Scribed by Philippa A. Coates; Ian S. Blagbrough; Terence Lewis; Barry V.L. Potter; Michael G. Rowan

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 280 KB
• Volume: 13
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(95)01599-x

✦ Synopsis

The extremely potent and selective nicotinic acctylcholine receptor antagonist mcthyllycaconitinc, M LA, and related norditerpenc alkaloids arc finding increasing use as neurochcmical probes and as targets for structure-activity relationship stutlics. In this work, an assay procedure for MLA which utiliscs ion suppression rcvcrse-ph:tse I IPLC with UV absorbancc detection at 270 nm is described. The method detected 2811 ng M LA on column.