An Expression Cloning Method to Identify Monomeric GTP-Binding Proteins by GTP Overlay

A cDNA Cloning Method for Monomeric GTP-

A cDNA Cloning Method for Monomeric GTP-Binding Proteins by Ligand Blotting

✍ Y. Nagano; R. Matsuno; Y. Sasaki 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 262 KB

We have developed a convenient method of cDNA cloning for monomeric GTP-binding proteins. The method consists of a serial dilution of cDNA expression library, a separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and a ligand blotting; each group containin