𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An evaluation of selected DNA extraction strategies for short tandem repeat typing

✍ Scribed by Dr. Nicholas Vandenberg; Roland. A. H. van Oorschot; Robert J. Mitchell


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
317 KB
Volume
18
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis


Abstract

Organic and Chelex extraction methods are compared to five commercially available DNA extraction kits. For forensic purposes, comparison of different extraction strategies is based on the ability of the methods to economically recover high yields of amplifiable DNA from small blood samples in a quick, simple and safe manner. The findings of this study indicate that resin‐based methods, such as Chelex, are best able to meet these criteria.


πŸ“œ SIMILAR VOLUMES


A simple Duplex-PCR to evaluate the DNA
✍ Nicole von Wurmb-Schwark; Thorsten Schwark; Michaela Harbeck; Manfred Oehmichen πŸ“‚ Article πŸ“… 2004 πŸ› Elsevier Science 🌐 English βš– 411 KB

Typing of DNA from ancient or otherwise highly degraded material, e.g. formalin fixed tissues, can be difficult, time consuming and costly. Very often, genetic typing is not possible at all. We present an inexpensive and easy to use Duplex-PCR that amplifies a 164 bp fragment specific for nuclear DN

Evaluation and validation of the ABI 370
✍ Pieris Koumi; Helen E. Green; Susan Hartley; Darren Jordan; Sharon Lahec; Richar πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 256 KB

The demand for high-throughput DNA profiling has increased with the introduction of national DNA databases and has led to the development of automated methods of short tandem repeat (STR) profile production; however, a potential bottleneck still exists at the gel electrophoresis stage. Capillary ele