An Evaluation of Factors Affecting the Elution of Antibodies from Bloodstains
โ Scribed by P.J. Lincoln; Barbara E. Dodd
- Publisher
- Elsevier
- Year
- 1973
- Tongue
- English
- Weight
- 689 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0015-7368
No coin nor oath required. For personal study only.
โฆ Synopsis
The principles inuolued in the reaction between antigen and antibody are considered and with these in mind, the work presented attempts to evaluate some of the factors afecting the elution of antibodies from bloodstains. Relevant factors such as the rate of association, the concentration of reactants, the ionic strength of the medium, the ejiciency of the elution procedure and the sensitivity of the detection of the eluted antibody have been investigated.
Using the modijications suggested, high titres of antibody can be recovered from minute amounts of stain.
๐ SIMILAR VOLUMES
## RG7 4PN, England Antibodies to specijc parasites may be readily detected in eluates of dried bloodstains. The method is based upon the reaction of a serum antibody with an antigen on a microscope slide. The presence of a positive reaction is visualized by JEuorescence microscopy after reacting
A rapid enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG antibodies against a cytoplasmic antigen of Candida albicans. The optimum conditions and time required for each step were investigated. Pre-equilibration at 37 degrees C and constant agitation of immunoreactants resulted i
The viscosity profiles of four different IgG 1 molecules were studied as a function of concentration at pH 6.0. At high concentrations, MAb-H and -A showed significantly higher viscosities as compared to MAb-G and -E. Zeta Potential (j) measurements showed that all the IgG 1 molecules carried a net