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An Escherichia coli host strain useful for efficient overproduction of secreted recombinant protein

✍ Scribed by Christian Weikert; Uwe Sauer; James E. Bailey

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
142 KB
Volume
59
Category
Article
ISSN
0006-3592

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✦ Synopsis

Periplasmic secretion of overexpressed Bacillus stearothermophilus ␣-amylase was analyzed in batch and fed-batch cultivations of Escherichia coli MG1655:pCSS4-p and the mutant strain CWML2:pCSS4p. Under all conditions investigated, growth and product formation of MG1655:pCSS4-p were severely impaired by heterologous protein expression and/or processing, while E. coli CWML2:pCSS4-p was found to be more robust and to accumulate 2-to 3-fold higher maximum ␣amylase levels. While this strain is itself potentially interesting for applications, its properties also illustrate the potential of the selection procedure that was employed to obtain it from its progenitor MG1655 (Weikert, C.,

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