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An EPR Method for Studying Annexin-Biomembrane Interaction

✍ Scribed by F.M. Megli; M. Selvaggi; A. Delisi; E. Quagliariello

Book ID
102967567
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
780 KB
Volume
214
Category
Article
ISSN
0003-2697

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✦ Synopsis

Annexin p34 spin label has been used as a probe for studying annexin-membrane Ca-mediated interaction. The EPR spectrum of the purified probe is able to monitor the binding of the protein to phospholipid vesicles and isolated mitochondrial membranes, with increasing calcium concentration, as revealed by the calculated rotational correlation time. A novel mathematical approach is proposed to provide a direct estimation of annexin percentage binding to membranes, based on the correlation time calculated from experimental EPR spectra.

πŸ“œ SIMILAR VOLUMES

Annexin V membrane interaction: an elect
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The possible role of electrostatic interactions for membrane binding and pore formation of annexin V has been analysed on the basis of a simple dielectric model. It is suggested that the binding of phospholipids to annexin V is regulated, at least initially, by the protein's electrostatic potential.

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