An epitope on Ki antigen recognized by autoantibodies from lupus patients shows homology with the sv40 large t antigen nuclear localization signal
✍ Scribed by Yoshinari Takasaki; Tetsuro Yano; Kaoru Hirokawa; Ken Takeuchi; Soichiro Ando; Toshimitsu Takahashi; Kohichiro Shimada; Hiroshi Hashimoto
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 773 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Objective. Epitopes on Ki antigen were analyzed using synthetic peptides, including KILT, a 16-mer peptide with an amino acid sequence homologous to the SV40 large T antigen nuclear localization signal (SV40 T NLS).
Methods. In addition to KILT, 4 synthetic peptides, all potential epitopes on Ki antigen according to computer analysis, were prepared and tested for reactivity with 49 anti-Ki-positive lupus sera by enzymelinked immunosorbent assay.
Results. Eighteen sera reacted with KILT, but not with other peptides. The reaction of anti-Ki sera with KILT was specifically inhibited by recombinant Ki antigen. Eight of 49 anti-Ki sera reacted with a 7-mer synthetic peptide of SV40 T NLS, and the reaction was specifically inhibited by KILT.
Conclusion. The 16-mer Ki peptide containing the sequence homologous to the SV40 T NLS is one of the antigenic epitopes recognized by anti-Ki antibodies in lupus sera.
An autoantibody to the soluble nuclear antigen Ki was first reported by Tojo et a1 in 1981 (1). Those investigators detected anti-Ki antibodies in 11.8% of patients with systemic lupus erythematosus (SLE) by . .