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An enzyme immunofiltration assay useful for detecting human monoclonal antibody

โœ Scribed by Mark C. Glassy; Harold H. Handley; Patrick H. Cleveland; Ivor Royston

Book ID
118062217
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
349 KB
Volume
58
Category
Article
ISSN
0022-1759

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โœ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub