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An enzyme immunoassay screening test for the detection of total antinuclear antibodies

✍ Scribed by Napoleon M. Moncé Jr.; N. Niels Cappel; Ronald T. Bogusky

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
350 KB
Volume
5
Category
Article
ISSN
0887-8013

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✦ Synopsis

The enzyme immunoassay antinuclear antibody (EIA ANA) screening test method is a new assay format for the qualitative determination of antinuclear antibodies (ANAs) in human serum or plasma. This assay method collectively detects ANAs against double stranded DNA (dsDNA), SS-AIRo, SS-BILa, Scl-70, Sm, and SmlRNP antigens, along with serum positive for peripheral, homogeneous, speckled, nucleolar, and centromere

Key words:

patterns. This assay correlated well with data obtained with hemagglutination testsfor specific ANA antigens, with the indirect immunofluorescence (IFA) ANA HEp-2 test, and with the Critftidia fucifiae IFA test for anti-dsDNA. This new test procedure is both highly specific and sensitive and substantially decreases the time involved when screening large numbers of patient samples. enzyme-linked immunosorbent assays, autoantibodies, immunofluorescence, hemagglutination, autoimmune disease

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