An Enzyme-Based Fiber Optic Biosensor for Determination of D-Amino Acid in Serum

✍ Scribed by Z.J. Zhang; Z.L. Gong; W.B. Ma

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 227 KB
• Volume: 52
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1995.1076

✦ Synopsis

An enzyme-based fiber optic D-amino acid biosensor is reported. It is based on coimmobilizing D-amino acid oxidase and horseradish peroxidase (HRP) to inert matrix bovine albumin via glutaraldehyde. A new fluorimetric substrate, thiamine, is used to indicate the sensing processes. The effect of (\mathrm{pH}), thiamine concentration, and temperature have been studied. Under optimized conditions, the detection limit is (0.3 \mu \mathrm{g} / \mathrm{ml}), the linear range of (D)-amino acid concentration is from 0.8 to (15 \mu \mathrm{g} / \mathrm{ml}) with excellent reversibility and stability, and the recovery is between 97.8 and (110.0 %). The sensor can be used for at least 3 weeks at room temperature. It is practicable to determine D-amino acid in serum directly with satisfactory results. 1995 Academic Press. Inc.