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An enzymatic method for measuring serum mannitol and its use in hemodialysis patients

โœ Scribed by Eleftherios P. Diamandis; C. Linda Grass; Robert Uldall; David Mendelssohn; Dinesh Maini

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
543 KB
Volume
25
Category
Article
ISSN
0009-9120

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โœฆ Synopsis

We evaluated two chemical methods for quantifying mannitol in serum, based on the oxidation of mannitol by periodate, and measurement of the formaldehyde formed with chromotropic acid (colorimetry) or acetylacetone (fluorometry). We found interference in these methods by serum glycerol. Additionally, a high-performance liquid chromatography (HPLC) method was evaluated and found to be specific but impractical for routine use. We therefore, developed an enzymatic fluorometric procedure, based on the oxidation of mannitol by beta-NAD to fructose and NADH, in the presence of the enzyme mannitol dehydrogenase (MD). MD is not commercially available and was partially purified from cultures of Leuconostoc mesenteroides. This new method is specific, sensitive, simple, and accurate and is proposed as the method of choice for measuring mannitol in the serum of patients who received this sugar alcohol during routine hemodialysis treatment.

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