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An enzymatic immunoassay for insulin-like growth factor-I (IGF-I)

✍ Scribed by M.J. Khosravi; A. Diamandi; J. Mistry; G. Mathew

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
118 KB
Volume
28
Category
Article
ISSN
0009-9120

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✦ Synopsis

We used two l~ghly ~Ixx~ifie pob'dond anebedi~ one hnrao. b'dized in microtitmtion wells and the other labeled with horseradish perexidase. In a two-step way, standards or pre-dihted serum samples (25 J~L, lO0-foid in the zero standard) tint react with the capture antibody for 2 h, thon washed and ineubeted with the labeded anfibedy for 1 h. After wuhing, the onzymatic activity of bound complexes is quanti-

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