An enzymatic assay of inorganic phosphate in serum using nucleoside phosphorylase and xanthine oxidase
β Scribed by Jacobus P.J. Ungerer; Heila M. Oosthuizen; Siegbert H. Bissbort
- Book ID
- 115827718
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 502 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
We developed an assay for serum magnesium (Mg) by coupling phosphoglucomutase (EC 2.7.5.1) with glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The kinetic generation of NADPH by the action of the above two enzymes upon glucose-l-phosphate (Gl-P) and glucoso-l,6-diphosphate (G-1,6-P) was proportion
The concentration of inorganic phosphate (Pi) and activity of adenosine deaminase (ADA) in human serum were determined by measuring the anodic current of uric acid with a dialysis membrane-covered carbon electrode in the presence of purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOD) in