✦ LIBER ✦

An ENDOR and ESEEM study of the blue copper protein azurin

✍ Scribed by J.W.A. Coremans; M. van Gastel; O.G. Poluektov; E.J.J. Groenen; T. den Blaauwen; G. van Pouderoyen; G.W. Canters; H. Nar; C. Hammann; A. Messerschmidt

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 682 KB
Volume: 235
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(95)00114-j

No coin nor oath required. For personal study only.

✦ Synopsis

We report electron-nuclear double-resonance experiments on a single crystal of azurin at 95 GHz and electron-spin-echo envelope-modulation experiments on frozen solutions of azurin and of the H117G mutant at 9 GHz. The hyperfine and quadrupole tensors of the two remote nitrogens of the histidine ligands of copper are assigned and discussed. A third nucleus is found to contribute to the echo-modulation spectrum and this probably concerns an amide nitrogen of the peptide backbone.

📜 SIMILAR VOLUMES

Matrix-assisted laser desorption/ionizat

Matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry analysis of blue copper proteins. Azurin and mavicyanin

✍ Tsuyoshi Fukuo; Naoko Kubota; Kunishige Kataoka; Masami Nakai; Shinnichirou Suzu 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 58 KB 👁 2 views

Two copper proteins azurin-1 and azurin-2 were isolated from denitrifying bacteria Alcaligenes xylosoxidans GIFU1051, and the mass spectrometric analysis of the proteins were carried out by both matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI).

Paramagnetic NMR studies of blue and pur

Paramagnetic NMR studies of blue and purple copper proteins

✍ Urszula Kolczak; Jesús Salgado; Gregg Siegal; Matti Saraste; Gerard W. Canters 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB 👁 2 views

1 H-and 13 C-NMR spectroscopy is applied to investigate the Cu A and type 1 active sites of copper proteins in solution. The analysis of hyperfine shifted 1 H resonances allows the comparison of the electron spin density delocalization in the Cu A site of the wild-type soluble domains of various cyt

Protonation of the Copper(I) Form of the

Protonation of the Copper(I) Form of the Blue Copper Proteins Plastocyanin and Amicyanin – A Molecular Dynamics Study

✍ Christian Buning; Peter Comba 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 555 KB

An ESR and ENDOR study of the hexahelice

An ESR and ENDOR study of the hexahelicene anion radical

✍ Robert D Allendoerfer; Raymond Chang 📂 Article 📅 1971 🏛 Elsevier Science ⚖ 270 KB

Structure, strain, and reorganization en

Structure, strain, and reorganization energy of blue copper models in the protein

✍ Ulf Ryde; Mats H. M. Olsson 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 247 KB 👁 2 views

An ESR. and ENDOR. Study of the Radical

An ESR. and ENDOR. Study of the Radical Anions of Cyano- and Nitro-Monosubstituted Naphthalenes

✍ Peter Fürderer; Fabian Gerson 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 German ⚖ 410 KB

## Abstract ^1^H‐ and ^14^N‐coupling constants have been determined by ESR. and ENDOR. spectroscopy for the radical anions of 1‐ and 2‐cyano‐, and 1‐ and 2‐nitronaphthalene.