An electrospray ionization mass spectrometry study of copper adducts of protonated ubiquitin
✍ Scribed by Charles Q. Jiao; Ben S. Freiser; Scott R. Carr; Carolyn J. Cassady
- Book ID
- 103996797
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 382 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1044-0305
No coin nor oath required. For personal study only.
✦ Synopsis
A study of the addition of Cu(II) to a ubiquitin electrospray solution shows that the copper ion in the ubiquitin remains doubly charged and displaces two protons on the protonated protein molecule. This observation indicates a chelating bond between the protein and the Cu(I1) species. The addition of Cu(1) also was studied and significant intensity was observed for adducts with up to four Cu(1) species attached, with each Cu(1) bonded to one basic site on the protein.
📜 SIMILAR VOLUMES
Several ethyl pyruvate and dihydrocinchonidine adducts, formed by non-covalent interactions with alkali cations, have been identified for the first time using electrospray ionization mass and tandem mass spectrometry. This type of adduct may have an important role in asymmetric reactions of pyruvate
## Abstract The reactions of ethyl pyruvate with acetic anhydride and pyridine were studied by electrospray ionization mass spectrometry (ESI‐MS). Ethyl 2‐acetoxy‐2‐pyridiniumpropionate (1) (__m__/__z__ 238) resulting from the reaction of the acetylpyridinium cation with ethyl pyruvate, and the add