An electrogenic chloride pump in a zoological membrane

✍ Scribed by Gerencser, George A.; Purushotham, Karnam R.; Meng, Hong-Bin

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 628 KB
• Volume: 275
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/(sici)1097-010x(19960701)275:4<256::aid-jez2>3.0.co;2-o

✦ Synopsis

Two widely documented mechanisms of chloride transport across animal plasma membranes are anion-coupled antiport and sodium-coupled symport. No direct genetic evidence has yet been provided for primary active chloride transport despite numerous reports of cellular C1--stimulated ATF'ases coexisting, in the same tissue, with uphill chloride transport that could not be accounted for by the two common chloride transport processes. C1--stimulated ATPases are a common property of practically all animal cells, with the major location being of mitochondria1 origin. It also appears that the plasma membranes of animal cells are sites of C1--stimulated ATPase activity. Recent studies of Cl--stimulated ATPase activity and chloride transport in the same membrane system, including liposomes, suggest a mediation by the ATPase in net movement of chloride up its electrochemical gradient across animal plasma membranes. Further studies, especially from a molecular biological perspective, are required to confirm a direct transport role to plasma membrane-localized C1--stimulated ATPases.