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An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability

โœ Scribed by Junping Fan; Jie Heng; Shuyan Dai; Neil Shaw; Bei Zhou; Bo Huang; Zheng He; Ya Wang; Taijiao Jiang; Xuemei Li; Zhijie Liu; Xianping Wang; Xuejun C. Zhang


Book ID
116829300
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
833 KB
Volume
78
Category
Article
ISSN
1046-5928

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## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo