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An efficient method for the determination ofKmvalues for HCO3-of phosphoenolpyruvate carboxylase

โœ Scribed by H. Bauwe

Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
426 KB
Volume
169
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

One of the most serious problems in obtaining estimates of the K m values for HCO 3 (-) of phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) by measurement of initial rates at varying HCO 3 (-) is the impossibility of completely excluding any contaminating HCO 3 (-) . A method is proposed which has no need for the careful control of HCO 3 (-) /CO2 contamination. The kinetic data are obtained by the evaluation of progress curves of HCO 3 (-) consumption. The method is discussed and the K m values for HCO 3 (-) of PEPCase from several C4-species are presented.

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