An efficient method for in vitro labeling proteins to high specific radioactivity using 3H-NaBH4 in dimethylformamide

✍ Scribed by David T. Cheung; Paul D. Benya; Alan Gorn; Marcel E. Nimni

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 520 KB
• Volume: 116
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90323-7

✦ Synopsis

A method for labeling proteins or insoluble protein matrices to high specific radioactivity using 'H-NaBH, in anhydrous dimethylformamide without the presence of formaldehyde or other aldehydes and ketones is presented. Chemical analysis of the labeled proteins suggests that the labeling mechanism involves methylation of lysyl residues. The procedure is simple and the labeled protein products retain their size and charge characteristics.