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An Efficient Fmoc-SPPS Approach for the Generation of Thioester Peptide Precursors for Use in Native Chemical Ligation

✍ Scribed by Juan B. Blanco-Canosa; Philip E. Dawson


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
426 KB
Volume
47
Category
Article
ISSN
0044-8249

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✍ Andrew B. Clippingdale; Colin J. Barrow; John D. Wade 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB 👁 1 views

Established methodology for the preparation of peptide thioesters requires the use of t-butoxycarbonyl chemistry owing to the lability of thioester linkers to the nucleophilic reagents used in Fmoc solid phase peptide synthesis. Both the greater ease of use and the broad applicability of the method