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An efficient extraction method for quantitation of adenosine triphosphate in mammalian tissues and cells

✍ Scribed by Junji Chida; Kazuhiko Yamane; Tunetomo Takei; Hiroshi Kido

Book ID
111643221
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
271 KB
Volume
727
Category
Article
ISSN
0003-2670

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The analysis of deoxyribonucleoside 5'-triphosphates (dNTPs) in cell extracts by high-pressure liquid chromatography [C. Garrett , and D.V. Santi (1979) Anal. Biochem. 99, 268-273] requires the prior, selective degradation of ribonucleoside 5'-triphosphates ( rNTPs ) that are present in the extracts