An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify (E)- and (Z)-diastereomers of α-asarone and β-asarone from Acorus tatarinowii Schott

✍ Scribed by Daijie Wang; Yanling Geng; Lei Fang; Xikai Shu; Jianhua Liu; Xiao Wang; Luqi Huang

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 145 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100573

✦ Synopsis

Abstract

In this study, supercritical fluid extraction (SFE) was used to extract essential oil from Acorus tatarinowii Schott under the pressure of 25 MPa and temperature of 35°C. Two (E)‐ and (Z)‐diastereomers of α‐asarone and β‐asarone were separated and purified by high‐speed counter‐current chromatography (HSCCC) with a two‐phase solvent system composed of n‐hexane–ethyl acetate–methanol–water (1:0.2:1:0.3, v/v). The separation yielded a total of 39 mg of α‐asarone and 463 mg of β‐asarone from 1.0 g of essential oil in one‐step separation with the purity of 98.9 and 99.1%, respectively, as determined by HPLC. The chemical structures of these compounds were identified by EI‐MS, ^1^H‐, and ^13^C‐NMR.