A relatively simple and inexpensive method has been developed for the preparation of highly purified rabbit reticulocyte globin mRNA. After phenol extraction, polysomal RNA was chromatographed on Sigmacell type 38 cellulose and Sepharose 4B. The resulting mRNA preparation has a purity in excess of 9
An effective method for the synthesis of the cap structure of eukaryotic messenger ribonucleic acids
β Scribed by Takashi Kamimura; Yumi Osaki; Mitsuo Sekine; Tsujiaki Hata
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- French
- Weight
- 259 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
β¦ Synopsis
A new promising "capping agent" (x) was synthesized by use of a lipophilic amino protecting group and an activatable pyrophosphate protecting group.
This agent reacted homogeneously with 5;rnucleotides in the presence of AgN03 to afford the cap structures (m7G pppNu).
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