An economic volt-hour integrator, compatible with commercial and homemade electrophoresis power supplies
✍ Scribed by Malte Rutschmann; Eberhardt von Berg; Lothar Kuehn; Burkhardt Dahlmann
- Book ID
- 102987157
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 645 KB
- Volume
- 142
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
In isoelectric focusing systems-a technique widely used for separation and characterization of proteins-the value of the volt-hour integral required to achieve steady-state focusing conditions differs markedly for different proteins. A convenient method to measure the correct volt-hour integral for a given protein, thereby assuring intra-and interlaboratory reproducibility of experiments, can be performed with a volt-hour integrator. As commercially available integrators are compatible only with the power supply marketed by the same manufacturer, a volt-hour integrator was designed which can be used in conjunction with any commercial or homemade power supply. This simple, low-cost integrator is expected to be of interest to those researchers who have so far refrained from introducing volt-hour integration, due to incompatibility of commercial volt-hour integrators with their isoelectric focusing system. o 1984 Academic Press. Inc.