An autoradiographic study of RNA synthesis in isolated salivary glands of Drosophila hydei. II. Interferometric studies
✍ Scribed by Arthur W. Pollister
- Publisher
- John Wiley and Sons
- Year
- 1965
- Tongue
- English
- Weight
- 805 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0362-2525
No coin nor oath required. For personal study only.
✦ Synopsis
Interferometric measurements were made on sections of salivary glands mounted in oils of refractive indices 1.410, 1.460, and 1.500, estimating optical path differences as fringe displacement with a Leitz double-beam interferometer microscope. From these data the mean concentrations ( i n mg/cm2/micron) of 55 cells of three glands were computed as follows: karyoplasm, 0.027 i-0.0026; cytoplasm, 0.048 * 0.0024; nucleolus, 0.066 & 0.0041. From these concentrations it was computed, according to Maurer and Primbsch (Exp. Cell Res., 33: 8-18) that the correction factors for €our micron sections were: karyoplasm, 0.203; cytoplasm, 0.114; nucleolus, 0.083. The reciprocals of these fractions (5.0, 8.8, and 12.0) are the correction factors of Arnold (J. Morph., in press) by which measured silver grain densities on autoradiographs are multiplied to give corrected (i.e. potential) densities.