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An autoradiographic evaluation of the regeneration of corneal epithelium inBufo marinus

✍ Scribed by Grillo, R. S. ;Knowles, B. A. ;Cooperstein, D. F.


Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
519 KB
Volume
203
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

Epithelial regeneration following scraping of a central portion of corneal epithelium of the toad, Bufo marinus, was studied. Wound closure occurred by migration of a single layer of epithelial cells from adjacent noninjured areas. Autoradiography indicated that following wound closure at two days after scraping, DNA synthesis began in these epithelial cells covering the former scraped area, and was even more pronounced three days after scraping. At four days the former denuded area was covered by an epithelium two to four cells thick and by the fifth day regeneration was complete. These studies also indicated that mitotic time in this organism is rapid.


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