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An automatic, on-line glucose analyzer for feed-back control of fed-batch growth ofEscherichia coli

✍ Scribed by Gregory W. Luli; Steven M. Schlasner; Donald E. Ordaz; Marc Mason; William R. Strohl


Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
497 KB
Volume
1
Category
Article
ISSN
0951-208X

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✦ Synopsis


A computer-assisted on-line glucose analyzer was developed for feed-back control of cell growth. Using this system the glucose consumption rate for Escherichia coli was determined to be linear ---em -during batch culture at 0.37 g/hr. On-line feed-back control of glucose concentration at 1.520.5 g/L was used with fed-batch cultures to produce 31.2 g dry weight of E. coli cells/L in 12 h. -es