An automatic method for determination of urinary 3-methylhistidine: Normal values

✍ Scribed by Monika Neuhäuser; Peter Fürst

• Book ID: 102985405
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 663 KB
• Volume: 92
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90662-6

✦ Synopsis

A system for automatic analysis of urinary 3-methylhistidine is described. applying ionexchange chromatography and using an automatic sample injector, a motoric selector valve, and a diode programmer.

which controls the analytical system.

The method permits a sampling rate of 22 samples/day.

3-Methylhistidine was completely separated from histidine in 37 min whereas I-methylhistidine was eluted together with ammonia. The 3-methylhistidine concentration was linear up to 150 nmoliml and no appreciable sample interaction was found at automatic sequential runs. The error. in a single determination based on duplicate samples, was 4.61% and, in duplicated determinations, 3.26%. The mean urinary 3-methylhistidine output was 299.4 2 23.8 Fmoliday in 12 healthy females and 545.5 ? 35.2 wmoliday in 12 healthy males. The 3-methylhistidine excretion was significantly higher in males than in females, when expressed as the absolute daily output or as the estimated ratio to body weight. body surface area. or creatinine.