An automatic continuous-flow spotting/striping machine

✍ Scribed by John M. Beattie; James A. Demetriou

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 577 KB
• Volume: 92
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97192-9

✦ Synopsis

This paper presents the principle, design and application of an automatic continuous-flow spotting/striping machine. The apparatus was specifically designed to apply multiple organic solvent extracts automatically and simultaneously as spots or stripes to chromatography paper. The machine applied twelve 300~,ul volumes of a methanol-dichloromethane (4:l) solution to chromatography paper in 30 min as uniform and compact stripes. The dynamic action of a unique mechanical "heart-shaped" cam was responsible for obtaining uniform and compact stripes. A recovery range of 93-98% for [3H]aldosterone (in methanol-dichloromethane) was obtained after pumping the solution through Acidflex@ and polyethylene tubing. The remaining

[3H]aldosterone was removed by two lOO-,ul methanol-dichloromethane washes. Urine aldosterone values showed no significant differences when extracts were applied by the machine or manually. This machine reduces the manhours required for sample applications three-fold and reduces operator tension built up by long hours of tedious manual applications.