An automated method for discriminating aneugen- vs. clastogen-induced micronuclei
✍ Scribed by Dorothea K. Torous; Stephen D. Dertinger; Nikki E. Hall; Carol R. Tometsko
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 131 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
A flow cytometric (FCM) procedure for quantitating 10,000 total reticulocytes per blood sample. A secmicronucleated reticulocytes in mouse peripheral ond analysis was performed on each sample blood samples was evaluated for its ability to dis-whereby the propidium iodide associated fluorescriminate between aneugen-and clastogen-induced cent signals of 250 MN were collected and micronuclei (MN). In this experiment, BALB/c mice graphed as a single-parameter histogram. The histowere injected with 0.9% saline, the model clastogen gram statistic ''median channel'' was recorded for methyl methanesulfonate (100 mg/kg bw) or the each sample and provided a quantitative descripaneugen vincristine (0.2 mg/kg bw). Peripheral tion of MN distribution according to DNA content. blood samples were collected 48 hr after injection Cumulatively, the results of this study suggest that and were subsequently fixed and stained for flow 1) flow cytometry can be employed to measure the cytometric analysis. The staining method utilized incidence of MN resulting from clastogenic or aneu-FITC-conjugated anti-CD71 to differentially label re-genic activity, and 2) MN resulting from aneugens ticulocytes, and the nucleic acid dye propidium io-can be discriminated from those arising spontaneously dide to resolve erythrocyte populations with and or from clastogen treatment based on flow cytometric without micronuclei. The frequency of micronucle-analysis of DNA content.