An automated method for acetylcholinesterase kinetics

✍ Scribed by R. Anand; M.G. Gore; G.A. Kerkut

• Book ID: 102630102
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 323 KB
• Volume: 70
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90471-1

✦ Synopsis

An automated assay for acetylcholinesterase (EC 3.1.1.7.) has been developed based on the manual spectrophotometric method of Ellman et al. (1). This method was used to determine (a) the enzyme activity of an unknown sample and (b) the dependence of initial rates given by a fixed enzyme concentration on the substrate concentration. Methods to minimize possible enzyme modification by DTNB (2) are described. Finally a modification of the conventional autoanalyser procedure permitted rapid and reproducible enzyme kinetic analysis under various conditions. This helped to minimize the effects of possible enzyme inactivation at high dilutions especially when using crude enzyme preparations.