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An automated fluorescence assay for subnanogram quantities of protein in the presence of interfering material

✍ Scribed by Klaus Dieter Hammer; Klaus Nagel

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
514 KB
Volume
155
Category
Article
ISSN
0003-2697

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✦ Synopsis

An automated assay for measuring nanogram and subnanogram quantities of protein in microliter samples was developed with the fluorometric reagent &phthaldialdehyde/mercaptoethanol. Low molecular weight interfering substances were separated within the analysis by gel filtration. The technique allowed measurement of biological fluids without any sample pretreatment. The method presented proved to be linear within the range 1.2 ng to 1.4 rg with a standard deviation of f4.2%. The minimum detectable protein concentration was 1 rg/liter. Special care was taken to prevent any determination errors caused by losses of protein during adsorption to surfaces. The simple analytical apparatus constructed can be used for field studies and ran several weeks when continuously used for seawater analysis aboard ship.

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