An automated continuous assay of membrane-bound and soluble ATPases and related enzymes

✍ Scribed by Alfred Arnold; H.Uwe Wolf; Bernd P. Ackermann; Hermann Bader

• Book ID: 102628177
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 292 KB
• Volume: 71
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90029-4

✦ Synopsis

A simple, rapid, and precise method is described for the continuous automated determination of the activity of membrane-bound enzymes which deliberate inorganic phosphate, e.g.. ATPases and phosphatases. The characteristics of this method, which is based on the determination of liberated phosphate in the presence of nucleotides, are: (A) the enzyme reaction can be followed continuously during a certain period, thus providing a higher precision, as compared to other methods in which the enzyme reaction is measured by few distinct determinations: (B) the enzyme protein and other (membrane) proteins of the enzyme preparation have not to be removed during the continuous determination of enzyme activity because they remain solubilized after denaturation; and (C) low or moderate concentration of nonionic detergents do not disturb the reading of the absorbancy.