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An atomic absorption method for cation measurements in Kjeldahl digests of biological materials

✍ Scribed by Hisashi Sanui; Nello Pace

Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
589 KB
Volume
47
Category
Article
ISSN
0003-2697

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✦ Synopsis

For the accurate measurement of cations in biological materials, destruction of organic matter is generally recommended and desirable because of interferences produced by some of the substances present (1). In this laboratory, a wet digestion procedure employing concentrated nitric and perchloric acids (2,3) and a low-temperature procedure using elect.rically excited oxygen (4,5) have been used with good results.

Frequently, however, analyses require digestion of heterogeneous samples such as tissue homogenates, prepared diets, feces, and dried tissues for which large aliquots must be taken in order to minimize sampling errors. Under these conditions, ashing with electrically excited oxygen is often difficult' and ineffective (5), and the wet digestion procedure requires large amounts of concentrated nitric and perchloric acids, making it hazardous in routine use because of the highly corrosive acid fumes and the dangers of violent. reactions (1). In many cases, determination of total nitrogen is also desired, generally requiring Kjelclahl digestion of a separate aliquot of the sample. From many considerations, therefore, it, appeared that, a method permitting measurement of cations in Kjeldahl digests would be a useful technique in the analysis of many types of biological samples.

A serious difficulty with this proposed technique, however, has been the substantial depression of calcium absorbance produced in the generally used acetylene-air flame by sulfate ion (&lo), which is added in large amounts to the sample as sulfuric acid in the digestion procedure. This depression can bc minimized by using a flame of higher temperature such as is obtained by the use of nitrous oxide-acetylene, but such a flame markedly increases ionization interferences (11-13) and also requires

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