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An Assay for Simultaneous Multiple Determinations of Peptide Binding to MHC Class II Molecules

✍ Scribed by A.A. Manfredi; M.H. Yuen; M.A. Raftery; B.M. Contitronconi

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 594 KB
Volume: 211
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1268

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✦ Synopsis

Binding of positively charged radiolabeled synthetic peptides to human major histocompatibility complex class II DR molecules, purified by affinity chromatography from lymphoblastoid B cell lines of different haplotypes, is rapidly, quantitatively, and specifically assayed by selective adsorption of the complexes between peptide and DR molecules onto DEAE-cellulose paper disks. This assay can be used as a revealing system of the ability of unlabeled test peptides to competitively inhibit the binding between the radiolabeled peptide and the DR molecules, thus measuring the binding of the competitor peptides, irrespective of their charge properties, to different DR molecules.

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