An assay for heparin by decrease in color yield (DECOY) of a protein-dye-binding reaction
✍ Scribed by M.Yahiya Khan; Stuart A. Newman
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 500 KB
- Volume
- 187
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The interference by heparin and some related molecules with the well-known Bradford dye-binding assay for proteins is used as the basis of a rapid, sensitive method for the quantitation of these polysaccharides. Whereas the available methods for the assay of glycosaminoglycans have lacked specificity for sulfated polyanions in general and for heparin in particular, the procedure described here distinguishes among different uronic acid derivatives and, when performed in conjunction with heparinase digestion or cetylpyridinium chloride precipitation, can be used to determine heparin content of complex biological samples.