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An Aspergillus oryzae acetyl xylan esterase: Molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris

โœ Scribed by Takuya Koseki; Yozo Miwa; Takeshi Akao; Osamu Akita; Katsumi Hashizume


Book ID
118471421
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
249 KB
Volume
121
Category
Article
ISSN
0168-1656

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Biochemical characterization of recombin
โœ Takuya Koseki; Yozo Miwa; Shinya Fushinobu; Katsumi Hashizume ๐Ÿ“‚ Article ๐Ÿ“… 2005 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 295 KB

We engineered an acetyl xylan esterase (AwaxeA) gene from Aspergillus awamori into a heterologous expression system in Pichia pastoris. Purified recombinant AwAXEA (rAwAXEA) displayed the greatest hydrolytic activity toward alpha-naphthylacetate (C2), lower activity toward alpha-naphthylpropionate (