An arylβ-d-glucosidase of the aquatic fungusLagenidium giganteum, a parasite of mosquito larvae
✍ Scribed by T. M. McInnis; A. J. Domnas
- Book ID
- 104780259
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 404 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Lagenidium giganteum Couch, a watermold parasitic on mosquito larvae possesses an intracellular fl-D-glucosidase (fl-D-glucoside glucohydrolase, E. C. 3.2.1.21). The enzyme was purified 132 fold and, with the substrate p-nitrophenyl fi-D-glncoside, was shown to have the following properties: The pH of optimum enzyme stability and activity lay between 5.5 and 6.0, and the enzyme was inactivated at temperatures above 50~ The Km was 4.6• 10 5 M and the Arrhenius activation energy was 8.35 Kcal -mole -~. Elution from Sephadex G-200 gave an approximate molecular weight of 120 000. The enzyme was inhibited by Pb ~+, Ag e § and Hg ~+, by glucose and by p-chloromercuribenzoate. The latter inhibition was overcome by cysteine. Chromatographic studies demonstrated transferase as well as hydrolase properties.