An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture
✍ Scribed by Philip J. Lee; Paul J. Hung; Luke P. Lee
- Book ID
- 101723888
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 401 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Primary hepatocytes represent a physiologically relevant model for drug toxicity screening. Here, we created a biologically inspired artificial liver sinusoid with a microfluidic endothelial‐like barrier having mass transport properties similar to the liver acinus. This unit consisted of a cord of hepatocytes (50 × 30 × 500 µm) fed by diffusion of nutrients across the microfluidic endothelial‐like barrier from a convective transport vessel (10 nL/min). This configuration sustained rat and human hepatocytes for 7 days without an extracellular matrix (ECM) coating. Experiments with the metabolism mediated liver toxicant diclofenac showed no hepatotoxicity after 4 h and an IC~50~ of 334 ± 41 µM after 24 h. Biotechnol. Bioeng. 2007; 97: 1340–1346. © 2007 Wiley Periodicals, Inc.