An Artificial CuII complex with intriguing oxygen radical-quenching profile. X-ray structure, cytochrome C assay, and ESR study
✍ Scribed by Masami Otsuka; Honoo Satake; Satoru Murakami; Mitsunobu Doi; Toshimasa Ishida; Masakatsu Shibasaki; Yukio Sugiura
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 489 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0968-0896
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✦ Synopsis
A novel artificial peptide named HPH-Pep, comprising a pyridine and two histidine units, was synthesized. The HPH-Pep-Cu" complex had unique pentacoordinated structure as shown by X-ray crystallography and exhibited superoxidescavenging activity as indicated by ESR spectroscopy. The superoxide-quenching profile of HPH-Pep-Cu" was studied in detail by cytochrome c assay and ESR spin trapping and it was found that (1) HPH-Pep-Cu" did not scavenge hydrogen peroxide or hydroxyl radical and hence the scavenging activity was specific to superoxide, and (2) HPH-Pep-Cu" did not generate hydrogen peroxide upon scavenging superoxide.