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An aqueous two-phase system of dextran/hydroxypropyldextran as a model in adsorption studies of Sephadex® gels

✍ Scribed by K. Lampert; H. Determann


Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
204 KB
Volume
63
Category
Article
ISSN
1873-3778

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✦ Synopsis


To investigate this we used monoethyl diglycol ether as the ~eluant and :Se@hodlex LH-20 as the gel since it gives the strongest adsorption effects ,of *all the Se@a:adex gel@. Fig. 2 shows that phenol is eluted before water, both without retardartiion,, ~%~iiclln means well before the total volume of the column (t/'t). Table I gives (data :about :sonne more substances run with this eluant on Sephades LH-20. The basic ckitietion off tillale separation in this solvent should be that of size difference only., .as in common :geIl chromatography.


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