An application of tetrazolium (MTT) colorimetric assay for the screening of anti-herpes simplex virus compounds
β Scribed by Hitoshi Takeuchi; Masanori Baba; Shiro Shigeta
- Book ID
- 119114952
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 740 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0166-0934
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Herpes simplex virus type 1 (HSV-1) encodes a deoxyribonuclease that is frequently referred to as alkaline nuclease (AN) because of its elevated pH optimum. Studies with recombinant viruses which contain deletions in the HSV-1 gene encoding AN have indicated that this enzyme is required for efficien
A novel multiplex nested polymerase chain reaction (PCR) assay was designed and evaluated for routine diagnosis of herpes simplex virus (HSV) infections in patients with either putative HSV infection of the central nervous system or suspected HSV keratitis. Single-tube amplification of HSV type 1 (H