An apparatus for the determination of volatile analytes by stopped-flow injection analysis using an integrated fiber optic detector

Determination of urease activity by flow

Determination of urease activity by flow-injection analysis using an ammonium-selective optrode as the detector

✍ Hong Li; Otto S. Wolfbeis 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 450 KB

Urease activity is determined by a combination of flow-injection analysis with optical sensor detection. Enzymatic hydrolysis of urea at pH 7.14 yields ammonium ions, which are determined using a fully reversible optical fibre ammonia sensor. Urea is contained in a buffered carrier stream into which